The Mouse FOXO1 (Forkhead Box Protein O1) CLIA Kit is designed for the accurate detection of FOXO1 levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for research applications.FOXO1 is a transcription factor that plays a critical role in regulating various cellular processes, such as cell cycle progression, apoptosis, and metabolism. Dysregulation of FOXO1 has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.
Overall, the Mouse FOXO1 CLIA Kit provides researchers with a powerful tool for investigating the role of FOXO1 in different biological processes and diseases, ultimately contributing to advancements in biomedical research.
Product Name:
Mouse FOXO1 (Forkhead Box Protein O1) CLIA Kit
SKU:
MOES00398
Target:
Mouse FOXO1 (Forkhead Box Protein O1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FOXO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FOXO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FOXO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FOXO1. You can calculate the concentration of Mouse FOXO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
91-102
96-107
Average (%)
107
97
101
1:4
Range (%)
91-102
100-115
85-96
Average (%)
96
108
90
1:8
Range (%)
87-101
94-105
96-108
Average (%)
94
99
102
1:16
Range (%)
96-109
90-101
91-106
Average (%)
103
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.69
227.77
920.13
109.77
245.84
913.01
Standard deviation
8.98
17.49
103.79
11.25
27.61
76.24
C V (%)
8.92
7.68
11.28
10.25
11.23
8.35
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FOXO1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Mouse FOXO1 in samples. No significant cross-reactivity or interference between Mouse FOXO1 and analogues was observed.
