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Mouse Folate Receptor 1, Adult (FOLR1) ELISA Kit

SKU:
MODL00497
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35846
Sensitivity:
31pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
FBP,FOLR,FR-alpha,MOv18,Adult folate-binding protein,KB cells FBP,Ovarian tumor-associated antigen MOv18
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Folate Receptor 1, Adult (FOLR1) ELISA Kit

The Mouse Folate Receptor 1 (Adult) (FOLR1) ELISA Kit is specifically designed for the accurate and reliable detection of FOLR1 levels in mouse samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with consistent and reproducible results, making it a valuable tool for a variety of research applications.FOLR1 is a key receptor involved in the transport of folate into cells, playing a critical role in cell growth, division, and overall function.

Dysregulation of FOLR1 has been implicated in various diseases, including cancer, autoimmune disorders, and neurological conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Mouse FOLR1 ELISA Kit offers researchers a reliable and efficient method for studying the role of FOLR1 in health and disease, providing valuable insights into its potential as a therapeutic target.

Product Name:Mouse Folate Receptor 1, Adult (FOLR1) ELISA Kit
Product Code:MODL00497
Size:96 Assays
Target:FOLR1
Synonyms:FBP, FOLR, FR-alpha, MOv18, Adult folate-binding protein, KB cells FBP, Ovarian tumor-associated antigen MOv18
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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