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Mouse Fibronectin (FN) ELISA Kit

SKU:
MODL00495
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11276
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FN1,CIG,FINC,LETS,MSF,GFND2,Anastellin,Migration-Stimulating Factor,Cold-Insoluble Globulin,Large, External, Transformation-Sensitive Protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibronectin (FN) ELISA Kit

The Mouse Fibronectin (FN) ELISA Kit is a reliable and accurate tool for detecting fibronectin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, leading to consistent and trustworthy results for various research applications.Fibronectin is a vital glycoprotein that plays a crucial role in cell adhesion, migration, and proliferation. It is also involved in wound healing, tissue remodeling, and embryonic development. Dysregulation of fibronectin expression has been linked to various diseases, including cancer, fibrosis, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

The Mouse Fibronectin (FN) ELISA Kit provides researchers with a user-friendly and efficient solution for quantifying fibronectin levels in biological samples, enabling detailed investigations into the role of fibronectin in health and disease.

Product Name:Mouse Fibronectin (FN) ELISA Kit
Product Code:MODL00495
Size:96 Assays
Target:FN
Synonyms:FN1, CIG, FINC, LETS, MSF, GFND2, Anastellin, Migration-Stimulating Factor, Cold-Insoluble Globulin, Large, External, Transformation-Sensitive Protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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