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Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit

SKU:
MODL00484
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P36363
Sensitivity:
34.55pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
KGF,HBGF7,Keratinocyte Growth Factor,Heparin-binding growth factor 7
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit

The Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit is a reliable tool for the accurate measurement of FGF7 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides trustworthy and consistent results, making it perfect for a wide range of research applications.FGF7 is a key growth factor that plays a critical role in cell growth and tissue repair, particularly in the skin, lung, and digestive tract.

Its dysregulation has been linked to various diseases, including cancer and inflammatory disorders, highlighting the importance of studying FGF7 levels as a potential biomarker for these conditions.Overall, the Mouse FGF7 ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of FGF7 in health and disease, providing accurate and reproducible results to support their studies.

Product Name:Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit
Product Code:MODL00484
Size:96 Assays
Target:FGF7
Synonyms:KGF, HBGF7, Keratinocyte Growth Factor, Heparin-binding growth factor 7
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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