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Mouse Fibroblast Growth Factor 3 (FGF3) ELISA Kit

SKU:
MODL00481
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05524
Sensitivity:
5.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
HBGF3,INT2,Heparin-binding growth factor 3,Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog,INT-2 Proto-Oncogene Protein,Proto-oncogene Int-2
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 3 (FGF3) ELISA Kit

The Mouse Fibroblast Growth Factor 3 (FGF3) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of FGF3 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.FGF3 is a key signaling protein that plays a crucial role in cell growth, development, and tissue repair. It is involved in processes such as wound healing, embryogenesis, and organ development, making it a valuable biomarker for studying these biological processes and potential therapeutic interventions.

With its high sensitivity and specificity, the Mouse FGF3 ELISA Kit is an essential tool for researchers investigating the role of FGF3 in various physiological and pathological conditions, including cancer, developmental disorders, and tissue regeneration. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.

Product Name:Mouse Fibroblast Growth Factor 3 (FGF3) ELISA Kit
Product Code:MODL00481
Size:96 Assays
Target:FGF3
Synonyms:HBGF3, INT2, Heparin-binding growth factor 3, Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog, INT-2 Proto-Oncogene Protein, Proto-oncogene Int-2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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