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Mouse Fibroblast Growth Factor 18 (FGF18) ELISA Kit

SKU:
MODL00477
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O89101
Sensitivity:
40.73pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
ZFGF5
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 18 (FGF18) ELISA Kit

The Mouse Fibroblast Growth Factor 18 (FGF18) ELISA Kit is specially designed for the precise measurement of FGF18 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.FGF18 is an important growth factor that plays a critical role in various biological processes, including tissue development, wound healing, and bone regeneration.

It is also implicated in diseases such as cancer and arthritis, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.With its reliable performance and broad utility, the Mouse FGF18 ELISA Kit is a valuable tool for researchers seeking to investigate the role of FGF18 in physiological and pathological processes.

Product Name:Mouse Fibroblast Growth Factor 18 (FGF18) ELISA Kit
Product Code:MODL00477
Size:96 Assays
Target:FGF18
Synonyms:ZFGF5
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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