null

Mouse Fibroblast Growth Factor 13 (FGF13) ELISA Kit

SKU:
MODL00475
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70377
Sensitivity:
13.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
FHF2,Fibroblast Growth Factor Homologous Factor 2
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 13 (FGF13) ELISA Kit

The Mouse Fibroblast Growth Factor 13 (FGF13) ELISA Kit is carefully crafted for the accurate measurement of FGF13 levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers precise and consistent results, making it an invaluable tool for various research applications.FGF13 is a key player in cell growth and differentiation, as well as in tissue repair and development.

Its role in various physiological and pathological processes, including cancer and neurological disorders, makes it an important target for investigation and potential therapeutic interventions.Explore the intricacies of FGF13 biology and its implications in disease research with the Mouse Fibroblast Growth Factor 13 (FGF13) ELISA Kit. Elevate your research capabilities and uncover new insights with confidence and precision.

Product Name:Mouse Fibroblast Growth Factor 13 (FGF13) ELISA Kit
Product Code:MODL00475
Size:96 Assays
Target:FGF13
Synonyms:FHF2, Fibroblast Growth Factor Homologous Factor 2
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products