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Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

SKU:
MODL00474
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P61329
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
FGF12B,FHF1,Fibroblast Growth Factor Homologous Factor 1,Myocyte-Activating Factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

The Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit is specifically designed for the precise measurement of FGF12 levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.FGF12 is a key factor in regulating cell growth and differentiation, as well as in neuronal function.

Its role in various cellular processes makes it a crucial target for studying conditions such as cancer, cardiovascular diseases, and neuronal disorders. By utilizing this ELISA kit, researchers can gain valuable insights into FGF12 levels and better understand its implications in disease pathology and potential therapeutic interventions.

Product Name:Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit
Product Code:MODL00474
Size:96 Assays
Target:FGF12
Synonyms:FGF12B, FHF1, Fibroblast Growth Factor Homologous Factor 1, Myocyte-Activating Factor
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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