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Mouse Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit

SKU:
MODL00471
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P61148
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ECGF,AFGF,ECGFA,ECGFB,FGF-Alpha,FGFA,HBGF1,ECGFB,HBGF1,Acidic Fibroblast Growth Factor,heparin-binding growth factor 1,Endothelial Cell Growth Factor, Beta
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit

The Mouse Fibroblast Growth Factor 1 (acidic FGF1) ELISA Kit is a powerful tool for researchers looking to accurately detect and quantify levels of acidic FGF1 in mouse serum, plasma, or cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications.Acidic FGF1, also known as fibroblast growth factor 1, plays a crucial role in various biological processes, including cell growth, differentiation, and development.

Its dysregulation has been implicated in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for studying and potentially targeting these diseases.The Mouse Fibroblast Growth Factor 1 (acidic FGF1) ELISA Kit is an essential tool for researchers seeking to deepen their understanding of acidic FGF1 and its role in health and disease.

Product Name:Mouse Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit
Product Code:MODL00471
Size:96 Assays
Target:FGF1
Synonyms:ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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