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Mouse Fibroblast Activation Protein Alpha (FAPa) ELISA Kit

SKU:
MODL00451
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97321
Sensitivity:
13.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
APCE,SIMP,DPPIV,Seprase,170 kDa melanoma membrane-bound gelatinase,Post-proline cleaving enzyme,Serine integral membrane protease,Surface-expressed protease
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Fibroblast Activation Protein Alpha (FAPa) ELISA Kit

The Mouse Fibroblast Activation Protein Alpha (FAPA) ELISA Kit is specifically designed for the accurate measurement of FAPA levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are essential for a variety of research applications.FAPA is a key protein involved in fibroblast activation and plays a crucial role in tissue remodeling, wound healing, and inflammation. It is also implicated in diseases such as fibrosis, cancer, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.

The Mouse FAPA ELISA Kit from Assay Genie provides researchers with a powerful tool to accurately quantify FAPA levels in mouse samples, facilitating in-depth investigations into the role of FAPA in various physiological and pathological processes. With its robust performance and ease of use, this kit is an indispensable asset for any laboratory conducting research on fibroblast activation and related pathways.

Product Name:Mouse Fibroblast Activation Protein Alpha (FAPa) ELISA Kit
Product Code:MODL00451
Size:96 Assays
Target:FAPa
Synonyms:APCE, SIMP, DPPIV, Seprase, 170 kDa melanoma membrane-bound gelatinase, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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