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Mouse Fibrinogen Beta (FGb) ELISA Kit

SKU:
MODL00470
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8K0E8
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FG-B,Fibrinopeptide B
Reactivity:
Mouse
Sample Type:
Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Fibrinogen Beta (FGb) ELISA Kit

The Mouse Fibrinogen Beta (FGB) ELISA Kit is specially developed for the precise measurement of FGB levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it perfect for various research purposes.Fibrinogen Beta is a key component of the blood clotting process, playing a crucial role in hemostasis and wound healing.

Dysregulation of FGB levels has been linked to various health conditions, including cardiovascular diseases, inflammatory disorders, and thrombotic events, highlighting its importance as a biomarker for disease research and therapeutic development.

Product Name:Mouse Fibrinogen Beta (FGb) ELISA Kit
Product Code:MODL00470
Size:96 Assays
Target:FGb
Synonyms:FG-B, Fibrinopeptide B
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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