null

Mouse Ferroportin (FPN) ELISA Kit

SKU:
MODL00502
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JHI9
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC40A1,FPN1,HFE4,IREG1,MTP1,SLC11A3,Solute Carrier Family 40,Member 1,Iron-Regulated Transporter
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Ferroportin (FPN) ELISA Kit

The Mouse Ferroportin (FPN) ELISA Kit is specifically designed for the precise detection of ferroportin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing trustworthy and consistent results, making it perfect for various research applications.Ferroportin, also known as the iron export protein, plays a crucial role in iron homeostasis by facilitating the release of iron from cells into the bloodstream.

Dysregulation of ferroportin has been linked to various diseases, including anemia, hemochromatosis, and cancer. Thus, studying ferroportin levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Mouse Ferroportin (FPN) ELISA Kit is a valuable tool for researchers looking to explore the role of ferroportin in health and disease in mouse models.

Product Name:Mouse Ferroportin (FPN) ELISA Kit
Product Code:MODL00502
Size:96 Assays
Target:FPN
Synonyms:SLC40A1, FPN1, HFE4, IREG1, MTP1, SLC11A3, Solute Carrier Family 40,Member 1, Iron-Regulated Transporter
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products