The Mouse Fe-Ferritin CLIA Kit is a cutting-edge assay designed for the precise measurement of mouse ferritin levels in biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Ferritin is a key protein involved in iron metabolism, playing a vital role in storing and releasing iron in the body. Abnormal ferritin levels have been linked to several health conditions including iron overload disorders, anemia, and inflammatory diseases.
Therefore, monitoring ferritin levels can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of potential treatments.With its advanced technology and user-friendly protocols, the Mouse Fe-Ferritin CLIA Kit from Assay Genie is a valuable tool for researchers studying iron metabolism and related disorders in mouse models. Get reliable and insightful data with this innovative assay kit.
Product Name:
Mouse FE (Ferritin) CLIA Kit
SKU:
MOES00257
Target:
Mouse FE (Ferritin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FE. You can calculate the concentration of Mouse FE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
90-105
89-104
Average (%)
94
96
95
1:4
Range (%)
85-99
97-114
89-104
Average (%)
91
104
96
1:8
Range (%)
100-119
85-99
89-102
Average (%)
108
91
94
1:16
Range (%)
98-114
86-98
90-102
Average (%)
105
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
3.07
9.06
0.98
3.22
8.38
Standard deviation
0.09
0.29
0.6
0.12
0.31
0.53
C V (%)
8.74
9.45
6.62
12.24
9.63
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FE in samples. No significant cross-reactivity or interference between Mouse FE and analogues was observed.
