Mouse Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit
SKU:
MODL00448
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51162
Sensitivity:
23pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
GT,iBABP,I-BABP,I-BALB,I-BAP,ILBP,ILBP3,ILLBP,I-15P,Gastrotropin,Illeal Lipid-Binding Protein,Ileal Bile Acid Binding Protein,Intestinal 15 kDa protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Mouse Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit
The Mouse Fatty Acid Binding Protein 6 (Ileal FABP6) ELISA Kit is a reliable tool designed for the accurate quantification of FABP6 levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.FABP6, also known as the ileal fatty acid binding protein, plays a crucial role in lipid metabolism and transport in the intestine. It is involved in the absorption and transport of dietary fatty acids, bile acids, and cholesterol, making it a key player in maintaining lipid homeostasis.
Dysregulation of FABP6 has been associated with metabolic disorders such as obesity, diabetes, and inflammatory bowel diseases, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.Overall, the Mouse FABP6 ELISA Kit provides researchers with a valuable tool for investigating the physiological and pathological roles of FABP6 in lipid metabolism and related diseases, ultimately contributing to advancements in the field of metabolic research.
Product Name:
Mouse Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
