Mouse FAS/CD95 (Factor Related Apoptosis) ELISA Kit
The Mouse Factor-Related Apoptosis (FAS/CD95) ELISA Kit is a specialized assay designed for the quantitative determination of FAS/CD95 levels in mouse biological samples. FAS/CD95, a critical transmembrane receptor protein, is known for its pivotal role in orchestrating apoptotic cell death through the activation of apoptosis pathways. Dysregulation of FAS/CD95 signaling has been linked to various disease states, including autoimmune disorders, cancer, and neurodegenerative conditions. This ELISA kit enables researchers to accurately measure FAS/CD95 levels, providing valuable insights into apoptosis signaling pathways, cellular survival, and disease progression. By quantifying FAS/CD95 expression in mouse samples, researchers can deepen their understanding of apoptosis regulation, identify potential therapeutic targets, and evaluate treatment responses targeting FAS/CD95-mediated apoptosis.
With exceptional sensitivity and specificity, the Mouse FAS/CD95 ELISA Kit ensures precise and reproducible results, empowering researchers to conduct detailed investigations into apoptotic pathways and associated diseases with confidence. Manufactured under rigorous quality control standards, this kit delivers reliable performance and user-friendly operation, offering a superior solution for research endeavors focused on apoptosis and related cellular processes in mouse models.
Product Name:
Mouse FAS/CD95 (Factor Related Apoptosis) ELISA Kit
SKU:
AEES00680
Target:
Mouse FAS/CD95 (Factor Related Apoptosis)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FAS/CD95. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FAS/CD95, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FAS/CD95. You can calculate the concentration of Mouse FAS/CD95 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
87-99
91-106
Average (%)
94
93
97
1:4
Range (%)
85-99
86-97
91-106
Average (%)
92
91
97
1:8
Range (%)
85-98
89-104
87-102
Average (%)
90
96
95
1:16
Range (%)
86-101
89-100
89-101
Average (%)
93
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.58
3.65
0.44
1.5
3.93
Standard deviation
0.03
0.06
0.11
0.03
0.08
0.17
C V (%)
5.23
4.04
3.02
6.89
5.64
4.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FAS/CD95 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FAS/CD95 in samples. No significant cross-reactivity or interference between Mouse FAS/CD95 and analogues was observed.
