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Mouse Factor Related Apoptosis Ligand (FASL) ELISA Kit

SKU:
MODL00453
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41047
Sensitivity:
0.060ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD178,CD95L,CD95-L,FASLG,FASLG,APT1LG1,APT1-LG1,TNFSF6,Fas Antigen Ligand,TNF Superfamily Member 6,Tumor Necrosis Factor(ligand)Superfamily,Member 6
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Mouse Factor Related Apoptosis Ligand (FASL) ELISA Kit

The Mouse FASL (Factor-Related Apoptosis Ligand) ELISA Kit is a cutting-edge tool designed for the precise measurement of FASL levels in mouse serum, plasma, and cell culture supernatants. This kit is engineered with superior sensitivity and specificity, ensuring the consistent and accurate detection of FASL for a variety of research purposes.FASL is a key protein involved in apoptosis, the programmed cell death process crucial for maintaining tissue homeostasis and eliminating damaged cells.

Dysregulation of FASL has been linked to various diseases and conditions, including cancer, autoimmune disorders, and inflammatory diseases. The Mouse FASL ELISA Kit provides researchers with a valuable tool for investigating the role of FASL in these conditions and potential therapeutic interventions.Get your hands on the Mouse FASL ELISA Kit today and unlock the secrets of apoptosis regulation in mouse models with confidence and reliability.

Product Name:Mouse Factor Related Apoptosis Ligand (FASL) ELISA Kit
Product Code:MODL00453
Size:96 Assays
Target:FASL
Synonyms:CD178, CD95L, CD95-L, FASLG, FASLG, APT1LG1, APT1-LG1, TNFSF6, Fas Antigen Ligand, TNF Superfamily Member 6, Tumor Necrosis Factor(ligand)Superfamily,Member 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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