Mouse FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
The Mouse Fatty Acid Binding Protein 1 (FABP1) ELISA Kit is crafted to deliver precise quantitative analysis of FABP1 levels across various biological samples, focusing on its liver-specific expression. FABP1, a member of the fatty acid binding protein family, predominantly expressed in the liver, plays a pivotal role in intracellular fatty acid transport and metabolism, contributing to lipid signaling and metabolic functions. Known for its involvement in lipid homeostasis and hepatic lipid utilization, FABP1 serves as a critical modulator of fatty acid trafficking and storage in liver cells. Accurate measurement of FABP1 is crucial for exploring its intricate role in lipid metabolism, liver function, and metabolic disorders. By quantifying FABP1 levels in biological specimens, researchers can unravel its involvement in fatty acid homeostasis, energy balance, hepatocellular injury, and metabolic diseases such as non-alcoholic fatty liver disease (NAFLD) or alcoholic liver disease.
Understanding the dynamics of FABP1 expression is key to deciphering its potential as a biomarker for liver health and metabolic dysregulations. The Mouse FABP1 ELISA Kit by Assay Genie guarantees superior sensitivity, specificity, and reproducibility, enabling dependable and consistent results to elevate your research investigations. Manufactured under stringent quality control measures, this kit ensures robust performance and user-friendly procedures, making it an optimal selection for scientists delving into the intricate mechanisms of liver lipid metabolism and metabolic health. Opt for Assay Genie's FABP1 ELISA Kit to obtain accurate measurements of FABP1 levels and illuminate its critical functions in liver lipid metabolism and metabolic disorders.
Product Name:
Mouse FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
SKU:
AEES00243
Target:
Mouse FABP1 (Fatty Acid Binding Protein 1, Liver)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FABP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FABP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FABP1. You can calculate the concentration of Mouse FABP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
94-104
99-111
Average (%)
94
101
105
1:4
Range (%)
89-102
88-98
88-99
Average (%)
97
92
94
1:8
Range (%)
92-101
97-108
100-109
Average (%)
96
104
103
1:16
Range (%)
92-100
91-102
88-98
Average (%)
96
95
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-98
90
EDTA plasma (n=5)
97-105
102
Cell culture media (n=5)
97-106
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.44
1.13
4.9
0.48
1.01
4.37
Standard deviation
0.02
0.05
0.23
0.03
0.05
0.23
C V (%)
4.55
4.42
4.69
6.25
4.95
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FABP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FABP1 in samples. No significant cross-reactivity or interference between Mouse FABP1 and analogues was observed.
