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PE/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1] (AGEL0572)

SKU:
AGEL0572
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
$263 - $623
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Description

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PE/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1]

The Mouse F4/80 Monoclonal Antibody PE-Cyanine5.5 Conjugated (CI:A3-1) (AGEL0572) offered by Assaygenie is a potent tool for immunophenotyping and flow cytometry applications. This antibody is specifically designed to target the F4/80 antigen, a well-established marker for mouse macrophages. With its PE-Cyanine5.5 conjugation, this antibody allows for multiparameter flow cytometry analysis with high sensitivity and specificity.Macrophages are essential immune cells involved in various biological processes, including host defense, tissue repair, and immune regulation. The F4/80 antigen is a reliable marker for identifying and characterizing macrophages in murine tissues and experimental models. By utilizing the Mouse F4/80 Monoclonal Antibody PE-Cyanine5.

5 Conjugated (CI:A3-1) from Assaygenie, researchers can accurately detect and quantify mouse macrophages in diverse experimental settings.Additionally, this antibody offers excellent staining performance, ensuring reliable and reproducible results in flow cytometry experiments. Its high affinity and selectivity for the F4/80 antigen make it a valuable tool for studying macrophage populations in immunology, inflammation, cancer research, and beyond.For researchers seeking a reliable and efficient solution for analyzing mouse macrophages, the Mouse F4/80 Monoclonal Antibody PE-Cyanine5.5 Conjugated (CI:A3-1) (AGEL0572) from Assaygenie is an essential reagent that enables precise and detailed characterization of these critical immune cells.

Product Title:PE/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1]
SKU:AGEL0572
Size:25µg, 100µg
Clone No:CI:A3-1
Synonyms:Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2b, κ
Isotype Control:PE/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:Liquid
Conjugation:PE/Cyanine 5.5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q61549
Gene ID:13733
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

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