The Mouse F4/80 Monoclonal Antibody (AF647 Conjugated, CI:A3-1) from Assay Genie (AGEL0565) is a powerful tool for immunological research. Specifically designed for detecting the F4/80 antigen in mouse samples, this monoclonal antibody is conjugated to Alexa Fluor 647 for robust fluorescent detection. F4/80 is a cell surface marker primarily found on murine macrophages and is widely used in the study of macrophage biology, inflammation, and tissue homeostasis. With its high specificity and sensitivity, this antibody enables precise identification and localization of F4/80-expressing cells in various experimental settings.
This antibody undergoes rigorous validation to ensure consistent performance in applications such as immunofluorescence microscopy, flow cytometry, and immunohistochemistry. Researchers investigating the role of macrophages in immune responses, infection, cancer, and inflammatory diseases will benefit from the reliable performance of the Mouse F4/80 Monoclonal Antibody (AF647 Conjugated, CI:A3-1) from Assay Genie.
Genie Fluor 647 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
Genie Fluor647
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
Q61549
Gene ID:
13733
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.