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Mouse Ezrin (Ezr) ELISA Kit (MOEB1128)

SKU:
MOEB1128
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26040
ELISA Type:
Sandwich
Synonyms:
CVL, EZR, CVIL, Cytovillin 2, EZR, p81, VIL2, Villin 2, Cytovillin, cytovillin 2, DKFZp762H157, FLJ26216, p81, VIL2MGC1584, villin 2, ezrin, villin-2, Villin-2
Reactivity:
Mouse
€649
Frequently bought together:

Description

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Mouse Ezrin (Ezr) ELISA Kit

The Mouse Ezrin (EZR) ELISA Kit is a powerful tool for researchers looking to accurately quantify levels of Ezrin in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.Ezrin is a key protein involved in cell structure and function, playing a critical role in processes such as cell adhesion, migration, and signaling. Dysregulation of Ezrin has been implicated in various diseases, including cancer, inflammatory disorders, and neurological conditions, making it an important target for research and therapeutic development.

By using the Mouse Ezrin ELISA Kit, researchers can gain valuable insights into the role of Ezrin in disease pathology and potentially identify new therapeutic strategies. Get started on your research today with this cutting-edge ELISA kit from Assay Genie.

Product Name:Mouse Ezrin (Ezr) ELISA Kit
SKU:MOEB1128
Size:96T
Target:Mouse Ezrin (Ezr)
Synonyms:Cytovillin, Villin-2, p81, Vil2
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Mouse
Detection Range:31.2-2000pg/mL
Sensitivity:15.74pg/mL
Intra CV:Provided with the Kit
Inter CV:Provided with the Kit
Linearity:Provided with the Kit
Recovery:Provided with the Kit
Function:Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Uniprot:P26040
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant mouse Ezrin
Sub Unit:Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS. Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites (By similarity). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (PubMed:21745462). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX.
Research Area:Epigenetics
Subcellular Location:Apical cell membrane Peripheral membrane protein Cytoplasmic side Cell projection Cell projection Microvillus membrane Peripheral membrane protein Cytoplasmic side Cell projection Ruffle membrane Peripheral membrane protein Cytoplasmic side Cytoplasm Cell cortex Cytoplasm Cytoskeleton Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Microvillar peripheral membrane protein (cytoplasmic side). Localizes to cell extensions and peripheral processes of astrocytes (By similarity).
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; T-tubule; cytosol; lipid raft; membrane; apical plasma membrane; cytoplasm; nucleolus; intracellular; uropod; vesicle; myelin sheath; filopodium; focal adhesion; actin filament; actin cytoskeleton; ruffle; extrinsic to membrane; cell projection; cytoskeleton; microvillus membrane; apical part of cell; plasma membrane

Molecular Function:actin filament binding; protein domain specific binding; protein binding; cytoskeletal protein binding; protein complex binding; cell adhesion molecule binding; actin binding

Biological Process: actin filament bundle formation; regulation of cell shape; microvillus biogenesis; filopodium formation; receptor internalization; establishment and/or maintenance of apical/basal cell polarity

UniProt Code:P26040
NCBI GenInfo Identifier:83921618
NCBI Gene ID:22350
NCBI Accession:NP_033536.2
UniProt Secondary Accession:P26040,Q80ZT8, Q9DCI1,
UniProt Related Accession:P26040
Molecular Weight:69,407 Da
NCBI Full Name:ezrin
NCBI Synonym Full Names:ezrin
NCBI Official Symbol:Ezr
NCBI Official Synonym Symbols:p81; Vil2; R75297; AW146364
NCBI Protein Information:ezrin; villin 2; villin-2; cytovillin
UniProt Protein Name:Ezrin
UniProt Synonym Protein Names:Cytovillin; Villin-2; p81
Protein Family:Ezrin
UniProt Gene Name:Ezr
UniProt Entry Name:EZRI_MOUSE
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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