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Mouse Estrogen Receptor Alpha (ERa) ELISA Kit

SKU:
MODL00430
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19785
Sensitivity:
0.146ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ER-A1,ER,ESR,ESR1,ESRA,NR3A1,NR3-A1,Estrogen Receptor 1,Nuclear Receptor Subfamily 3,Group A,Member 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse Estrogen Receptor Alpha (ERa) ELISA Kit

The Mouse Estrogen Receptor Alpha (ERα) ELISA Kit is specifically designed for the precise quantification of estrogen receptor alpha levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit is known for its high level of sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.Estrogen receptor alpha is a critical protein involved in mediating the effects of estrogen in various physiological processes, including cell growth and differentiation.

Dysregulation of ERα has been linked to conditions such as breast cancer, osteoporosis, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With the Mouse Estrogen Receptor Alpha (ERα) ELISA Kit, researchers can confidently analyze ERα levels in mouse samples, providing valuable insights into the role of this important protein in health and disease.

Product Name:Mouse Estrogen Receptor Alpha (ERa) ELISA Kit
Product Code:MODL00430
Size:96 Assays
Target:ERa
Synonyms:ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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