The Mouse EPO (Erythropoietin) CLIA Kit is designed for the precise measurement of Erythropoietin levels in mouse serum, plasma, and tissue homogenates. This high-quality kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Erythropoietin is a key hormone involved in the regulation of red blood cell production, making it a critical factor in conditions such as anemia, kidney disease, and cancer treatment.
This kit provides researchers with a valuable tool for studying the role of Erythropoietin in these conditions and developing potential therapeutic interventions.Overall, the Mouse EPO CLIA Kit from Assay Genie is a reliable and efficient solution for researchers seeking to investigate the role of Erythropoietin in various physiological and pathological processes in mouse models.
Product Name:
Mouse EPO (Erythropoietin) CLIA Kit
SKU:
MOES00024
Target:
Mouse EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EPO. You can calculate the concentration of Mouse EPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
91-106
95-109
Average (%)
99
97
100
1:4
Range (%)
95-109
91-107
93-104
Average (%)
102
99
99
1:8
Range (%)
100-113
89-102
86-99
Average (%)
105
94
93
1:16
Range (%)
95-108
96-110
96-109
Average (%)
102
102
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.44
252.27
855.93
95.13
236.8
937.1
Standard deviation
11.39
23.46
85.51
9.17
18.45
90.9
C V (%)
11.81
9.3
9.99
9.64
7.79
9.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EPO in samples. No significant cross-reactivity or interference between Mouse EPO and analogues was observed.
