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Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

SKU:
MODL00409
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70424
Sensitivity:
0.207ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD340,ErbB2,HER-2/neu,HER2,NEU,MLN 19,NGL,TKR1,p185,V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2,Neuro/Glioblastoma Derived Oncogene Homolog
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

The Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of EGFR2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent specificity and reproducibility, making it a valuable tool for a wide range of research applications.EGFR2 is a critical receptor involved in cell growth, proliferation, and differentiation. Dysregulation of EGFR2 is associated with various diseases, including cancer and inflammatory disorders.

By accurately measuring EGFR2 levels, researchers can gain insights into disease mechanisms and potential therapeutic targets.This ELISA kit from Assay Genie provides researchers with a precise and efficient method for quantifying EGFR2 levels in mouse samples, allowing for valuable data in their studies. Trust in the reliability of this kit to advance your research on EGFR2 and its role in disease pathology.

Product Name:Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit
Product Code:MODL00409
Size:96 Assays
Target:EGFR2
Synonyms:CD340, ErbB2, HER-2/neu, HER2, NEU, MLN 19, NGL, TKR1, p185, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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