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Mouse EP2 (Prostaglandin E Receptor 2)ELISA Kit (MOES01397)

SKU:
MOES01397
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62053
Sensitivity:
0.09ng/mL
Range:
0.16-10ng/mL
ELISA Type:
Sandwich
Reactivity:
Mouse
Sample Type:
Serum, plasma and other biological fluids
€649
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Description

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Mouse EP2 (Prostaglandin E Receptor 2) ELISA Kit

The Mouse EP2 (Prostaglandin E Receptor 2) ELISA Kit is specifically designed for the accurate detection of EP2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.EP2 is a key receptor involved in the prostaglandin E signaling pathway, playing a crucial role in inflammation, immune response, and pain regulation. Dysregulation of EP2 has been associated with various diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With easy-to-follow instructions and quick assay procedures, the Mouse EP2 ELISA Kit provides researchers with a reliable tool to investigate the role of EP2 in different biological processes and disease pathways. Whether studying inflammation, immune disorders, or pain management, this kit offers a versatile solution for accurate EP2 detection and quantification.

Assay type:Sandwich
Format:96T
Assay time:4.5h
Reactivity:Mouse
Detection Method:Colormetric
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Volume Required Per Well:100µL
Sample Type:Serum, plasma and other biological fluids
Specificity:This kit recognizes Mouse EP2 in samples. No significant cross-reactivity or interference between Mouse EP2 and analogues was observed.

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EP2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EP2. The concentration of Mouse EP2 in samples can be calculated by comparing the OD of the samples to the standard curve.

UniProt Protein Function:PTGER2: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Cellular Component: integral to membrane; membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity; prostaglandin E receptor activity; signal transducer activity

Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; regulation of cell proliferation; response to lipopolysaccharide; response to progesterone stimulus; signal transduction

UniProt Code:Q62053
NCBI GenInfo Identifier:6679529
NCBI Gene ID:19217
NCBI Accession:NP_032990. 1
UniProt Related Accession:Q62053
Molecular Weight:40,479 Da
NCBI Full Name:prostaglandin E2 receptor EP2 subtype
NCBI Synonym Full Names:prostaglandin E receptor 2 (subtype EP2)
NCBI Official Symbol:Ptger2
NCBI Official Synonym Symbols:EP2; Ptgerep2
NCBI Protein Information:prostaglandin E2 receptor EP2 subtype
UniProt Protein Name:Prostaglandin E2 receptor EP2 subtype
UniProt Synonym Protein Names:Prostanoid EP2 receptor
Protein Family:Prostaglandin E2 receptor
UniProt Gene Name:Ptger2
UniProt Entry Name:PE2R2_MOUSE

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e. g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(ng/mL)		O.DAverageCorrected
102.455
2.513		2.4842.403
51.686
1.732		1.7091.628
2.50.933
0.903		0.9180.837
1.250.498
0.518		0.5080.427
0.630.265
0.263		0.2640.183
0.320.192
0.174		0.1830.102
0.160.128
0.14		0.1340.053
00.074
0.088		0.081--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse EP2 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse EP2 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)0.511.373.740.511.243.97
Standard deviation0.030.060.190.030.060.18
C V (%)5.884.385.085.884.844.53

Recovery

The recovery of Mouse EP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)87-10092
EDTA plasma (n=5)93-109100
Cell culture media (n=5)91-10398

Linearity

Samples were spiked with high concentrations of Mouse EP2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)99-11096-10890-105
Average (%)10410297
1:4Range (%)93-11085-10088-100
Average (%)1009193
1:8Range (%)89-10184-9487-101
Average (%)968892
1:16Range (%)88-10186-9784-97
Average (%)959290

An unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 µL
Concentrated HRP Conjugate (100×) 1 vial, 120 µL -20°C(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4°C(shading light)
Stop Solution 1 vial, 10 mL 4°C
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy
  1. Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
  2. Aliquot 100µl of standard solutions into the standard wells.
  3. Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
  4. Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
  5. Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
  6. Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix. Incubate for 1 hour at 37°C.
  7. Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
  8. Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
  9. Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
  10. Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
  11. Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
  12. Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.

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