null

Mouse Enolase, Neuron Specific (NSE) ELISA Kit

SKU:
MODL00970
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17183
Sensitivity:
0.069ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ENO2,Enolase 2,Gamma Enolase,2-phospho-D-glycerate hydro-lyase,Neural enolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Enolase, Neuron Specific (NSE) ELISA Kit

The Mouse Enolase Neuron-Specific (NSE) ELISA Kit is specifically designed for the precise measurement of NSE levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.NSE is a neuron-specific enzyme that plays a crucial role in neuronal function and survival. It is a valuable biomarker for monitoring neuronal damage and assessing neurological disorders such as stroke, traumatic brain injury, and neurodegenerative diseases.

This ELISA kit is essential for studying the pathophysiology of these conditions and developing potential therapeutic interventions.Don't miss out on the opportunity to accurately measure NSE levels in mouse samples with our reliable and easy-to-use ELISA kit. Upgrade your research capabilities with the Mouse Enolase Neuron-Specific (NSE) ELISA Kit from Assay Genie.

Product Name:Mouse Enolase, Neuron Specific (NSE) ELISA Kit
Product Code:MODL00970
Size:96 Assays
Target:NSE
Synonyms:ENO2, Enolase 2, Gamma Enolase, 2-phospho-D-glycerate hydro-lyase, Neural enolase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products