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Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit

SKU:
MODL00435
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70351
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KMT6B,ENX-2,Histone-lysine N-methyltransferase EZH1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit

The Mouse Enhancer of Zeste Homolog 1 (EZH1) ELISA Kit is specifically designed for the quantitative measurement of EZH1 levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and accuracy, ensuring precise and consistent results for various research applications.EZH1 is a key component of the polycomb repressive complex 2 (PRC2) and plays a critical role in regulating gene expression and chromatin structure. Dysregulation of EZH1 has been implicated in various diseases, including cancer, developmental disorders, and neurological conditions.

Therefore, measuring EZH1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its reliable performance and ease of use, the Mouse EZH1 ELISA Kit is an essential tool for researchers studying epigenetics, gene regulation, and disease progression in mouse models. Order yours today and unlock new possibilities in your research.

Product Name:Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit
Product Code:MODL00435
Size:96 Assays
Target:EZH1
Synonyms:KMT6B, ENX-2, Histone-lysine N-methyltransferase EZH1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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