Mouse ELANE Recombinant Protein (RPPB1611)
- SKU:
- RPPB1611
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q3UP87
- Research Area:
- Enzymes
Description
| Product Name: | Mouse ELANE Recombinant Protein |
| Product Code: | RPPB1611 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | ELANE |
| Synonyms: | Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastase. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ELANE protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 30% Glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAHDLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGDRTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPLVCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTNH HHHHH |
Elastase-2 (ELANE) is a serine protease that belongs to the chymotrypsin family.�ELANE breaks down elastin, an elastic fiber together with collagen and controls the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. ELANEis inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin.A1AT is usually secreted by the liver cells into the serum. A1AD leads to uninhibited destruction of elastic fiber by elastase.
ELANE Mouse produced in Sf9 Insect cells is a single, glycosylated polypeptide chain (27-265a.a.) fused to a 9 aa His Tag at C-terminus containing 245 amino acids and having a molecular mass of 26.8kDa.ELANE shows multiple bands between 28-40kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.21.37; Cell cycle regulation; Motility/polarity/chemotaxis; Cell surface Cellular Component: cell surface; cytoplasm; extracellular space; secretory granule; transcriptional repressor complex Molecular Function:cytokine binding; endopeptidase activity; heparin binding; hydrolase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity Biological Process: acute inflammatory response to antigenic stimulus; defense response to bacterium; defense response to fungus; leukocyte migration; leukocyte migration during inflammatory response; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of immune response; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to lipopolysaccharide; response to UV; response to yeast |
| NCBI Summary: | This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q3UP87 |
| NCBI GenInfo Identifier: | 114052444 |
| NCBI Gene ID: | 50701 |
| NCBI Accession: | NP_056594.2 |
| UniProt Related Accession: | Q3UP87 |
| Molecular Weight: | |
| NCBI Full Name: | neutrophil elastase |
| NCBI Synonym Full Names: | elastase, neutrophil expressed |
| NCBI Official Symbol: | Elane�� |
| NCBI Official Synonym Symbols: | NE; Ela2; F430011M15Rik�� |
| NCBI Protein Information: | neutrophil elastase |
| UniProt Protein Name: | Neutrophil elastase |
| UniProt Synonym Protein Names: | Elastase-2; Leukocyte elastase |
| UniProt Gene Name: | Elane�� |
| UniProt Entry Name: | ELNE_MOUSE |
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