The Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit is expertly crafted for the precise and quantitative measurement of EGFR levels in a variety of biological samples.
EGFR, known for its crucial role as a transmembrane receptor tyrosine kinase, is a key player in diverse cellular processes such as cell proliferation, survival, differentiation, and migration. Signaling through EGFR regulates fundamental cellular functions and pathways, making it a significant target for research in various fields. Accurately measuring EGFR levels is essential for gaining insights into its involvement in growth factor signaling, cancer progression, and other physiological and pathological processes. Understanding the expression and activity of EGFR can provide valuable information for studying its impact on cell behavior, disease development, and therapeutic interventions. The Mouse EGFR ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Produced under rigorous quality control measures, this kit delivers robust performance and user-friendly features, making it an excellent choice for researchers investigating the functions and implications of Epidermal Growth Factor Receptor in biological systems.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EGFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EGFR. You can calculate the concentration of Mouse EGFR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
92-107
88-99
Average (%)
91
99
93
1:4
Range (%)
85-98
89-102
84-98
Average (%)
91
96
90
1:8
Range (%)
89-102
96-108
86-98
Average (%)
94
102
93
1:16
Range (%)
82-96
89-103
84-96
Average (%)
89
95
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.12
47.06
146.64
17.69
42.37
151.93
Standard deviation
1.08
2.43
4.78
0.89
1.86
6.41
C V (%)
5.66
5.16
3.26
5.02
4.4
4.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse EGFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EGFR in samples. No significant cross-reactivity or interference between Mouse EGFR and analogues was observed.
