The Mouse EGF (Epidermal Growth Factor) CLIA Kit is a highly sensitive assay designed for the accurate detection of EGF levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring consistent and trustworthy results for a variety of research applications.EGF is a vital growth factor that plays a pivotal role in regulating cell growth, proliferation, and differentiation. Its involvement in crucial biological processes such as wound healing, tissue regeneration, and development makes it a valuable biomarker for studying various diseases and conditions.
With its high performance and reliability, the Mouse EGF CLIA Kit is an essential tool for researchers looking to investigate the role of EGF in physiological and pathological processes, opening up new avenues for therapeutic interventions and advancements in the field of biomedicine.
Product Name:
Mouse EGF (Epidermal growth factor) CLIA Kit
SKU:
MOES00022
Target:
Mouse EGF (Epidermal growth factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EGF. You can calculate the concentration of Mouse EGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
87-99
85-97
Average (%)
101
93
90
1:4
Range (%)
103-119
91-102
94-108
Average (%)
110
97
100
1:8
Range (%)
90-106
88-103
94-108
Average (%)
97
96
99
1:16
Range (%)
87-101
101-111
92-108
Average (%)
94
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.39
57.38
186.35
23.87
61.49
170.42
Standard deviation
2.86
6.35
14.55
1.97
5.36
11.78
C V (%)
11.73
11.07
7.81
8.25
8.72
6.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EGF in samples. No significant cross-reactivity or interference between Mouse EGF and analogues was observed.
