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Mouse Dystrophin Associated Glycoprotein 1 (DAG1) ELISA Kit

SKU:
MODL00351
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62165
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Dystroglycan 1,156DAG,A3a,AGRNR,DAG,Alpha-Dystroglycan,Beta-Dystroglycan
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Dystrophin Associated Glycoprotein 1 (DAG1) ELISA Kit

The Mouse Dystrophin-Associated Glycoprotein 1 (DAG1) ELISA Kit from AssayGenie is a specialized assay designed for the precise measurement of DAG1 levels in mouse samples such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs.DAG1 is a critical component of the dystrophin-associated glycoprotein complex involved in muscle cell membrane stability and function. Mutations in the DAG1 gene can lead to muscular dystrophy and other neuromuscular disorders, making it a crucial target for understanding these conditions and developing potential treatments.

By utilizing the Mouse DAG1 ELISA Kit, researchers can gain valuable insights into the role of DAG1 in muscle health and disease, aiding in the advancement of therapeutic strategies for muscular dystrophy and related disorders. Trust AssayGenie for reliable and high-quality ELISA kits for your research endeavors.

Product Name:Mouse Dystrophin Associated Glycoprotein 1 (DAG1) ELISA Kit
Product Code:MODL00351
Size:96 Assays
Target:DAG1
Synonyms:Dystroglycan 1, 156DAG, A3a, AGRNR, DAG, Alpha-Dystroglycan, Beta-Dystroglycan
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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