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Mouse Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) ELISA Kit (MOEB0864)

SKU:
MOEB0864
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31938
ELISA Type:
Sandwich
Reactivity:
Mouse
€649
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Description

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Mouse Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) ELISA Kit

The Mouse Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit is specifically designed for the quantitative measurement of MAP2K1 levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.MAP2K1, also known as MEK1, is a key enzyme in the MAPK signaling pathway, regulating cell growth, differentiation, and survival. Dysregulation of MAP2K1 has been implicated in various diseases, including cancer, inflammatory disorders, and cardiovascular diseases.

Therefore, this ELISA kit serves as a valuable tool for studying MAP2K1 signaling pathways and developing potential therapeutic interventions.Overall, the Mouse Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit provides researchers with a precise and efficient method for analyzing MAP2K1 levels in mouse samples, contributing to a better understanding of the molecular mechanisms involved in various physiological and pathological processes.

Product Name:Mouse Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) ELISA Kit
SKU:MOEB0864
Size:96T
Target:Mouse Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1)
Synonyms:ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP kinase kinase 1, Mek1, Prkmk1
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Mouse
Detection Range:0.156-10ng/mL
Sensitivity:0.059ng/mL
Intra CV:Provided with the Kit
Inter CV:Provided with the Kit
Linearity:
Sample1:21:41:81:16
Serum(N=5)100-108%103-111%104-114%103-113%
EDTA Plasma(N=5)92-102%96-109%109-118%84-84%
Heparin Plasma(N=5)93-102%109-119%106-118%96-107%
Recovery:Provided with the Kit
Function:Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Uniprot:P31938
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant mouse Dual specificity mitogen-activated protein kinase kinase 1
Sub Unit:Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3/ERK1 and RGS14. Forms heterodimers with KSR2 which further dimerize to form tetramers. Interacts with ARRB2, LAMTOR3, MAPK1/ERK2, RAF1, PPARG AND VRK2. Interacts with SGK1, BIRC6/bruce (By similarity). Interacts with KSR-1 (PubMed:10409742). Interacts with MORG1 (PubMed:15118098). Forms a heterodimer with MAP2K2/MEK2 (PubMed:19219045).
Research Area:Cancer
Subcellular Location:Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Cytoplasm Cytoskeleton Microtubule organizing center Spindle pole body Cytoplasm Nucleus Membrane Peripheral membrane protein Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (By similarity). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742).
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 familyCellular Component: axon; cell cortex; cytoplasm; cytosol; dendrite; dendrite cytoplasm; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule; mitochondrion; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membraneMolecular Function: ATP binding; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine/tyrosine kinase activity; Ras GTPase binding; receptor signaling protein serine/threonine kinase activity; receptor signaling protein tyrosine phosphatase activityBiological Process: activation of MAPK activity; Bergmann glial cell differentiation; cell cycle arrest; cell motility involved in cell locomotion; cell proliferation; cerebellar cortex formation; Golgi inheritance; heart development; keratinocyte differentiation; MAPKKK cascade; melanosome transport; mitosis; negative regulation of cell proliferation; negative regulation of homotypic cell-cell adhesion; neurite morphogenesis; neuron differentiation; positive regulation of axonogenesis; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of Ras protein signal transduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; regulation of axon regeneration; regulation of protein amino acid phosphorylation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; response to axon injury; response to glucocorticoid stimulus; response to oxidative stress; thymus development; thyroid gland development; vesicle transport along microtubule
UniProt Protein Details:
NCBI Summary:
UniProt Code:P31938
NCBI GenInfo Identifier:400275
NCBI Gene ID:26395
NCBI Accession:P31938.2
UniProt Secondary Accession:P31938
UniProt Related Accession:P31938
Molecular Weight:43,474 Da
NCBI Full Name:Dual specificity mitogen-activated protein kinase kinase 1
NCBI Synonym Full Names:mitogen-activated protein kinase kinase 1
NCBI Official Symbol:Map2k1
NCBI Official Synonym Symbols:Mek1; MEKK1; MAPKK1; Prkmk1
NCBI Protein Information:dual specificity mitogen-activated protein kinase kinase 1
UniProt Protein Name:Dual specificity mitogen-activated protein kinase kinase 1
UniProt Synonym Protein Names:ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1
Protein Family:
UniProt Gene Name:Map2k1
UniProt Entry Name:MP2K1_MOUSE
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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