The Mouse DPP4 (Dipeptidyl Peptidase Ⅳ) ELISA Kit is a cutting-edge assay designed to quantitatively measure the levels of DPP4, also known as CD26, in mouse biological samples. DPP4 is a multifunctional serine protease involved in numerous physiological processes, including glucose homeostasis, immune regulation, and signal transduction. By accurately detecting and quantifying DPP4 levels, this ELISA kit offers valuable insights into the role of DPP4 in various biological pathways and disease conditions. This highly sensitive ELISA kit enables researchers to investigate the expression and activity of DPP4, providing valuable data for studies on metabolic disorders, immune responses, and inflammation.
DPP4 has emerged as a potential therapeutic target for conditions such as diabetes, cancer, and immunological disorders, making this ELISA kit an indispensable tool for understanding DPP4 biology and its implications in health and disease. With exceptional sensitivity and specificity, the Mouse DPP4 ELISA Kit from Assay Genie ensures accurate and reproducible results. Manufactured under stringent quality control standards, this kit guarantees robust performance and ease of use, making it a top choice for researchers conducting studies on DPP4 in mouse models.
Product Name:
Mouse DPP4 (Dipeptidyl Peptidase Ⅳ) ELISA Kit
SKU:
AEES00659
Target:
Mouse DPP4 (Dipeptidyl Peptidase Ⅳ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DPP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DPP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DPP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DPP4. You can calculate the concentration of Mouse DPP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
99-111
95-110
Average (%)
104
105
102
1:4
Range (%)
97-111
99-115
98-112
Average (%)
104
105
104
1:8
Range (%)
100-113
99-113
95-109
Average (%)
106
107
102
1:16
Range (%)
98-115
98-111
95-112
Average (%)
105
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.08
12.83
42.4
5.42
11.68
43.18
Standard deviation
0.34
0.76
1.45
0.37
0.7
1.31
C V (%)
6.65
5.89
3.43
6.82
5.99
3.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse DPP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DPP4 in samples. No significant cross-reactivity or interference between Mouse DPP4 and analogues was observed.
