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Mouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

SKU:
MODL00357
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JLM8
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
DCDC3A,DCLK,DCAMKL1,Doublecortin And CaM Kinase-Like 1,Doublecortin domain-containing protein 3A,Doublecortin-like and CAM kinase-like 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

The Mouse Doublecortin-Like Kinase 1 (DCLK1) ELISA Kit is a powerful tool for the precise measurement of DCLK1 levels in mouse samples including serum and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring trustworthy and consistent results for various research endeavors.DCLK1 is an important kinase that plays a critical role in cell differentiation, proliferation, and survival, particularly in the context of cancer and gastrointestinal disorders.

By accurately measuring DCLK1 levels, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets.With its high-quality components and reliable performance, the Mouse DCLK1 ELISA Kit is an indispensable asset for investigating the functions of DCLK1 and its implications in various pathologies. Choose this kit for precise and reliable quantification of DCLK1 levels in mouse samples.

Product Name:Mouse Doublecortin Like Kinase 1 (DCLK1) ELISA Kit
Product Code:MODL00357
Size:96 Assays
Target:DCLK1
Synonyms:DCDC3A, DCLK, DCAMKL1, Doublecortin And CaM Kinase-Like 1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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