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Mouse Discoidin Domain Receptor Family, Member 1 (DDR1) ELISA Kit

SKU:
MODL00362
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q03146
Sensitivity:
0.047ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD167a,CAK,DDR,EDDR1,MCK10,NEP,NTRK4,PTK3,PTK3A,RTK6,TRKE,Cell adhesion kinase,Epithelial discoidin domain receptor 1,Mammary carcinoma kinase 10
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Discoidin Domain Receptor Family, Member 1 (DDR1) ELISA Kit

The Mouse Discoidin Domain Receptor Family Member 1 (DDR1) ELISA Kit is a powerful tool for accurately measuring DDR1 levels in mouse samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.DDR1 is a key receptor involved in cell adhesion and migration, playing a critical role in processes such as tissue repair and inflammation.

Dysregulation of DDR1 has been linked to diseases such as cancer, fibrosis, and arthritis, making it an important target for further study.By using the Mouse DDR1 ELISA Kit, researchers can gain valuable insights into the role of DDR1 in health and disease, potentially leading to the development of novel therapeutic strategies. Give your research an edge with this cutting-edge ELISA kit from Assay Genie.

Product Name:Mouse Discoidin Domain Receptor Family, Member 1 (DDR1) ELISA Kit
Product Code:MODL00362
Size:96 Assays
Target:DDR1
Synonyms:CD167a, CAK, DDR, EDDR1, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE, Cell adhesion kinase, Epithelial discoidin domain receptor 1, Mammary carcinoma kinase 10
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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