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Mouse Dipeptidyl Peptidase IV (DPP4) ELISA Kit

SKU:
MODL00387
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28843
Sensitivity:
5.2pg/mL
Range:
12.5-800pg/mL
ELISA Type:
Sandwich
Synonyms:
CD26,ADA,DPPIV,DPP-IV,ADABP,ADCP2,TP103,Adenosine Deaminase Complexing Protein 2,T-cell activation antigen CD26
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Dipeptidyl Peptidase IV (DPP4) ELISA Kit

The Mouse Dipeptidyl Peptidase IV (DPP4) ELISA Kit from Assay Genie is a highly sensitive and specific kit designed for the accurate detection of DPP4 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Dipeptidyl Peptidase IV (DPP4) is an important enzyme involved in various biological processes, including glucose metabolism, immune regulation, and inflammation.

Dysregulation of DPP4 activity has been linked to conditions such as diabetes, obesity, and inflammatory diseases, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse DPP4 ELISA Kit from Assay Genie is a valuable tool for researchers looking to accurately measure DPP4 levels in mouse samples, helping to advance our understanding of the role of DPP4 in health and disease.

Product Name:Mouse Dipeptidyl Peptidase IV (DPP4) ELISA Kit
Product Code:MODL00387
Size:96 Assays
Target:DPP4
Synonyms:CD26, ADA, DPPIV, DPP-IV, ADABP, ADCP2, TP103, Adenosine Deaminase Complexing Protein 2, T-cell activation antigen CD26
Detection Method:Sandwich
Range:12.5-800pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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