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Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit

SKU:
MODL00388
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q80YA7
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DP8,DPRP1,MSTP141,MSTP097,MSTP135,dipeptidylpeptidase 8,Dipeptidyl Peptidase IV-Related Protein-1,Prolyl Dipeptidase DPP VIII
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit

The Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit is a powerful tool for the precise measurement of DPP8 levels in mouse serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a variety of research studies.DPP8 is a vital enzyme that plays a key role in regulating various biological processes, including immune response and inflammation. Dysregulation of DPP8 has been linked to the development of certain diseases, making it a valuable target for further investigation and potential therapeutic interventions.

With the Mouse DPP8 ELISA Kit, researchers can confidently analyze DPP8 levels in mouse samples, allowing for a better understanding of its role in health and disease. This kit is a valuable resource for advancing scientific knowledge and uncovering new insights into the functions of DPP8 in biological systems.

Product Name:Mouse Dipeptidyl Peptidase 8 (DPP8) ELISA Kit
Product Code:MODL00388
Size:96 Assays
Target:DPP8
Synonyms:DP8, DPRP1, MSTP141, MSTP097, MSTP135, dipeptidylpeptidase 8, Dipeptidyl Peptidase IV-Related Protein-1, Prolyl Dipeptidase DPP VIII
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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