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Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

SKU:
MODL00378
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08749
Sensitivity:
0.08ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LDH-D,DLD,Probable D-lactate dehydrogenase, mitochondrial
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

The Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit is designed for the quantitative detection of DLD levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications. Dihydrolipoyl dehydrogenase is a key enzyme involved in the citric acid cycle and plays a critical role in energy metabolism. Dysregulation of DLD has been linked to a variety of diseases, including metabolic disorders and neurodegenerative conditions.

Therefore, measuring DLD levels can provide valuable insights into these pathologies and aid in the development of potential therapeutic interventions. With easy-to-follow instructions and quick assay procedures, the Mouse DLD ELISA Kit is an essential tool for researchers studying mouse models of disease and investigating the molecular mechanisms underlying metabolic dysfunction. Order your kit today and accelerate your research efforts.

Product Name:Mouse Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit
Product Code:MODL00378
Size:96 Assays
Target:DLD
Synonyms:LDH-D, DLD, Probable D-lactate dehydrogenase, mitochondrial
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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