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Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit

SKU:
MODL00370
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z2A7
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ARGP1,DGAT,ARAT,ACAT-related gene product 1,Acyl-CoA retinol O-fatty-acyltransferase,Diglyceride acyltransferase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit

The Mouse Diacylglycerol O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit is specifically designed for the accurate and sensitive detection of DGAT1 levels in mouse serum, plasma, and tissue lysates. This kit offers high specificity and precision, ensuring reliable and reproducible results for various research applications.DGAT1 is an enzyme that plays a key role in lipid metabolism, specifically in the synthesis of triglycerides. Dysregulation of DGAT1 has been linked to obesity, diabetes, and other metabolic disorders, making it a valuable target for research and drug development.

By accurately quantifying DGAT1 levels, researchers can gain insights into lipid metabolism and potentially identify novel therapeutic strategies for these conditions.Overall, the Mouse DGAT1 ELISA Kit provides researchers with a powerful tool to study the role of DGAT1 in health and disease, ultimately advancing our understanding of lipid metabolism and metabolic disorders.

Product Name:Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit
Product Code:MODL00370
Size:96 Assays
Target:DGAT1
Synonyms:ARGP1, DGAT, ARAT, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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