Mouse Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit
The Mouse Defensin Alpha 5 (Paneth Cell Specific) ELISA Kit is specifically designed for the accurate detection of Defensin Alpha 5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results, making it an excellent choice for a variety of research applications.Defensin Alpha 5 is a key protein produced by Paneth cells in the intestine, playing a significant role in the innate immune response by targeting and killing harmful bacteria.
Dysregulation of Defensin Alpha 5 levels has been linked to various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With easy-to-follow protocols and quick assay times, the Mouse Defensin Alpha 5 ELISA Kit from Assay Genie is an essential tool for researchers investigating the role of Defensin Alpha 5 in the immune response and gastrointestinal health in mice.
Product Name:
Mouse Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit
Product Code:
MODL00365
Size:
96 Assays
Target:
DEFa5
Synonyms:
DEF-A5, DEF5, HD-5
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
