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Mouse Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit

SKU:
MODL00364
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11477
Sensitivity:
0.56ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
DEF1,DEFA2,HNP-1,HP-1,MRS,DEF3,HNP-3,HNP3,HP-3,Defensin,Alpha 1,Myeloid-Related Sequence
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit

The Mouse Defensin Alpha 1 (Neutrophil DEFA1) ELISA Kit is specifically designed for the quantitative measurement of DEFA1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it perfect for a variety of research applications.Defensin Alpha 1 is an important component of the immune system, playing a key role in defending against bacterial infections and inflammation.

Its levels are known to be altered in various disease states, making it a valuable biomarker for studying immune responses and developing new therapeutic strategies.Overall, the Mouse Defensin Alpha 1 (Neutrophil DEFA1) ELISA Kit provides a valuable tool for researchers looking to better understand the role of DEFA1 in immune function and disease pathogenesis.

Product Name:Mouse Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit
Product Code:MODL00364
Size:96 Assays
Target:DEFa1
Synonyms:DEF1, DEFA2, HNP-1, HP-1, MRS, DEF3, HNP-3, HNP3, HP-3, Defensin,Alpha 1, Myeloid-Related Sequence
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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