The Mouse DEFA5 (Defensin Alpha 5) CLIA Kit is specifically designed for the quantitative measurement of DEFA5 levels in Paneth cells of mouse tissue samples. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for their studies.Defensin Alpha 5 is a key antimicrobial peptide produced by Paneth cells in the small intestine, playing a crucial role in protecting against bacterial infections and maintaining gut homeostasis. Dysregulation of DEFA5 expression has been associated with various gastrointestinal disorders, making it a valuable biomarker for investigating these conditions and developing targeted therapies.
With its user-friendly protocol and robust performance, the Mouse DEFA5 CLIA Kit is an essential tool for researchers studying the role of Paneth cells and antimicrobial peptides in gut health and disease. Whether analyzing gene expression patterns or evaluating therapeutic interventions, this kit provides the precision and accuracy needed for meaningful research outcomes.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFa5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DEFa5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DEFa5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DEFa5. You can calculate the concentration of Mouse DEFa5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
97-111
92-105
Average (%)
96
102
98
1:4
Range (%)
94-105
87-101
84-97
Average (%)
99
92
91
1:8
Range (%)
100-116
94-105
95-106
Average (%)
108
99
101
1:16
Range (%)
98-116
94-111
95-110
Average (%)
106
102
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
3.18
8.41
1.0
3.38
8.52
Standard deviation
0.09
0.24
0.86
0.09
0.34
0.64
C V (%)
9.38
7.55
10.23
9.0
10.06
7.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DEFa5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DEFa5 in samples. No significant cross-reactivity or interference between Mouse DEFa5 and analogues was observed.
