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Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

SKU:
MODL00311
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JM58
Sensitivity:
5.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CISS,CISS1,CLF,CLF-1,NR6,Cytokine-like factor 1,ZcytoR5
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

The Mouse Cytokine Receptor-Like Factor 1 (CRLF1) ELISA Kit is specifically designed for the accurate detection of CRLF1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.CRLF1 is an important cytokine receptor-like factor that plays a key role in immune response regulation and inflammation. Its involvement in various diseases and conditions makes it a valuable biomarker for studying the underlying mechanisms and potential therapeutic strategies.

With the Mouse CRLF1 ELISA Kit, researchers can confidently analyze CRLF1 levels in mouse samples, leading to a better understanding of its function and implications in disease pathology. Its reliability and performance make it a valuable tool for advancing research in immunology and related fields.

Product Name:Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit
Product Code:MODL00311
Size:96 Assays
Target:CRLF1
Synonyms:CISS, CISS1, CLF, CLF-1, NR6, Cytokine-like factor 1, ZcytoR5
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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