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Mouse Cytokine Array (22 targets) (SARB0078)

SKU:
SARB0078
Product Type:
Protein Array
Analytes:
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
IL-13
IL-17A
IFN-gamma
MCP-1 (CCL2)
MCP-5
RANTES (CCL5)
SCF
TNF RI (TNFRSF1A)
TNF alpha
Thrombopoietin (TPO)
VEGF-A
Reactivity:
Mouse
Applications:
Multiplex Array
€559 - €1,089
Frequently bought together:

Description

system_update_altDatasheet

Mouse Cytokine Array (22 targets) (SARB0078)

The Mouse Cytokine Array (SAR-B0078) is a comprehensive research tool designed for the simultaneous detection and quantification of 22 cytokine targets in mouse samples. This array enables researchers to analyze cytokine levels in a high-throughput and cost-effective manner, providing valuable insights into the immune response and inflammatory processes.Each array contains a panel of capture antibodies immobilized on a solid support, allowing for the specific binding of target cytokines present in the sample. The array can be used with a variety of sample types, including serum, plasma, cell culture supernatants, and tissue lysates, making it versatile for different experimental needs.

Whether studying inflammatory diseases, immune responses, or cancer biology, the Mouse Cytokine Array offers a powerful tool for researchers looking to profile cytokine expression patterns and identify potential therapeutic targets. With its high sensitivity and reproducibility, this array is a valuable asset for any laboratory conducting cytokine profiling studies in mouse models.

Product Sku:SARB0078
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:22
Gene Symbols:CCL12, CCL2, CCL5, CSF2, CSF3, IFNG, IL10, IL12A, IL13, IL17A|IL2, IL3, IL4, IL5, IL6, IL9, KITLG, THPO, TNF, TNFRSF1A, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
GCSF
  • Gene Symbol: CSF3
  • Gene ID: N/A
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: N/A
  • Accession #: P26955
IL-2
  • Gene Symbol: IL2
  • Gene ID: N/A
  • Accession #: Q791T3
IL-3
  • Gene Symbol: IL3
  • Gene ID: N/A
  • Accession #: P01586
IL-4
  • Gene Symbol: IL4
  • Gene ID: N/A
  • Accession #: P07750
IL-5
  • Gene Symbol: IL5
  • Gene ID: N/A
  • Accession #: P04401
IL-6
  • Gene Symbol: IL6
  • Gene ID: N/A
  • Accession #: P08505
IL-9
  • Gene Symbol: IL9
  • Gene ID: N/A
  • Accession #: P15247
IL-10
  • Gene Symbol: IL10
  • Gene ID: N/A
  • Accession #: Q0VBJ1
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Gene ID: N/A
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: N/A
  • Accession #: P43431
IL-13
  • Gene Symbol: IL13
  • Gene ID: N/A
  • Accession #: P20109
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: N/A
  • Accession #: Q60971
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: N/A
  • Accession #: P01580
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: N/A
  • Accession #: Q9QYD7
MCP-5
  • Gene Symbol: Ccl12
  • Gene ID: N/A
  • Accession #: Q9QYD6
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: N/A
  • Accession #: P30882
SCF
  • Gene Symbol: KITLG
  • Gene ID: N/A
  • Accession #: P20826
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: N/A
  • Accession #: P25118
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: N/A
  • Accession #: Q62326
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: N/A
  • Accession #: P40226
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: N/A
  • Accession #: Q00731
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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