Mouse Cytochrome C Oxidase Subunit III (COX3) ELISA Kit
The Mouse Cytochrome C Oxidase Subunit III (COX3) ELISA Kit is a powerful tool for accurately measuring levels of COX3 in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides precise and consistent results, making it perfect for a wide range of research applications.COX3 is a critical subunit of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain. It plays a key role in electron transfer and ATP production, making it essential for cellular respiration and energy metabolism.
Abnormalities in COX3 expression have been linked to various disorders, including mitochondrial diseases, neurodegenerative disorders, and aging-related conditions.By using the Mouse COX3 ELISA Kit, researchers can gain valuable insights into mitochondrial function, oxidative metabolism, and the impact of COX3 dysregulation on health and disease. This kit is a valuable tool for studying mitochondrial dysfunction and developing potential therapeutic strategies for related disorders.
Product Name:
Mouse Cytochrome C Oxidase Subunit III (COX3) ELISA Kit
Product Code:
MODL00298
Size:
96 Assays
Target:
COX3
Synonyms:
MTCO3, MT-CO3, COIII, COXIII, Mitochondrially Encoded Cytochrome C Oxidase III
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
