The Mouse Cys-C (Cystatin C) ELISA Kit is a specialized assay designed for the sensitive and quantitative detection of Cystatin C levels in mouse samples. Cystatin C serves as a valuable biomarker for assessing renal function and is commonly utilized in clinical and research settings to evaluate kidney health and function. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results essential for researchers studying kidney diseases, renal function, and related pathologies.
Manufactured under strict quality control standards, the kit provides reliable performance and ease of use, making it an optimal choice for investigative and diagnostic studies on renal health and associated conditions.
Product Name:
Mouse Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00219
Target:
Mouse Cys-C (Cystatin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Cys-C. You can calculate the concentration of Mouse Cys-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-103
95-105
88-96
Average (%)
96
101
93
1:4
Range (%)
85-95
92-101
87-98
Average (%)
91
97
90
1:8
Range (%)
96-110
96-108
84-94
Average (%)
104
101
90
1:16
Range (%)
93-99
92-106
87-101
Average (%)
96
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-102
99
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.88
105.09
456.05
40.09
123.09
409.82
Standard deviation
2.55
4.76
20.89
2.29
5.97
17.95
C V (%)
5.22
4.53
4.58
5.71
4.85
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Cys-C in samples. No significant cross-reactivity or interference between Mouse Cys-C and analogues was observed.
