The Mouse CypA (Cyclophilin A) ELISA Kit is specifically designed for the accurate measurement of mouse CypA levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Cyclophilin A is an important protein that plays a role in various cellular processes, including protein folding and immune response regulation.
It has been implicated in inflammatory diseases, viral infections, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Mouse CypA ELISA Kit is a valuable tool for researchers looking to measure and analyze CypA levels in mouse samples with precision and accuracy.
Product Name:
Mouse CYPA (Cyclophilin A) ELISA Kit
SKU:
MOES00939
Target:
Mouse CYPA (Cyclophilin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CYPA. You can calculate the concentration of Mouse CYPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
84-97
95-108
Average (%)
104
91
101
1:4
Range (%)
87-101
85-101
83-93
Average (%)
94
92
88
1:8
Range (%)
91-105
85-97
85-98
Average (%)
96
92
90
1:16
Range (%)
87-101
88-102
81-93
Average (%)
93
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.04
4.31
0.58
0.94
4.4
Standard deviation
0.03
0.05
0.21
0.04
0.04
0.22
C V (%)
5.56
4.81
4.87
6.9
4.26
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CYPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYPA in samples. No significant cross-reactivity or interference between Mouse CYPA and analogues was observed.
