The Mouse CT (Calcitonin) CLIA Kit is specifically developed for the precise measurement of calcitonin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Calcitonin is a vital hormone that plays a key role in regulating calcium levels in the body and bone metabolism. It is also involved in various physiological processes, including bone development and maintenance.
Abnormal calcitonin levels have been associated with conditions such as osteoporosis, hypercalcemia, and Paget's disease, making it a valuable biomarker for investigating these diseases and exploring potential treatments.Overall, the Mouse CT (Calcitonin) CLIA Kit provides researchers with a reliable tool to investigate the role of calcitonin in various biological processes and diseases, offering valuable insights for advancing scientific knowledge and potential therapeutic strategies.
Product Name:
Mouse CT (Calcitonin) CLIA Kit
SKU:
MOES00136
Target:
Mouse CT (Calcitonin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CT. You can calculate the concentration of Mouse CT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
94-108
102-114
Average (%)
104
102
108
1:4
Range (%)
89-103
97-109
86-98
Average (%)
97
102
93
1:8
Range (%)
100-111
100-112
84-98
Average (%)
105
106
90
1:16
Range (%)
99-112
94-109
91-105
Average (%)
105
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
100-113
107
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.39
62.25
226.77
26.33
65.32
240.69
Standard deviation
2.37
5.05
15.67
2.71
5.83
23.64
C V (%)
9.72
8.11
6.91
10.29
8.93
9.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CT in samples. No significant cross-reactivity or interference between Mouse CT and analogues was observed.
