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Mouse Crk Like Protein (CRKL) ELISA Kit

SKU:
MODL00310
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P47941
Sensitivity:
33pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Crk Like Protein (CRKL) ELISA Kit

The Mouse CRK-Like Protein (CRKL) ELISA Kit is a reliable and precise tool for measuring CRKL levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for a variety of research purposes.CRKL is a key protein that plays a critical role in cell signaling pathways and is involved in various cellular processes such as cell growth, differentiation, and migration.

Dysregulation of CRKL has been linked to various diseases, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse CRK-Like Protein (CRKL) ELISA Kit offers a valuable tool for researchers looking to study the role of CRKL in different biological processes and disease states. Its reliable performance and ease of use make it a must-have for any laboratory conducting research in this area.

Product Name:Mouse Crk Like Protein (CRKL) ELISA Kit
Product Code:MODL00310
Size:96 Assays
Target:CRKL
Synonyms:V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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