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Mouse Corticosteroid Binding Globulin (CBG) ELISA Kit

SKU:
MODL00217
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06770
Sensitivity:
2.47ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
SERPINA6,Transcortin,Serpin A6,Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase,Antitrypsin)
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Corticosteroid Binding Globulin (CBG) ELISA Kit

The Mouse Corticosteroid-Binding Globulin (CBG) ELISA Kit is specifically designed for the accurate quantification of CBG levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of applications.CBG is a key protein that binds to corticosteroids, playing a crucial role in regulating the activity of these hormones.

Dysregulation of CBG levels has been associated with various health conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.With the Mouse Corticosteroid-Binding Globulin (CBG) ELISA Kit, researchers can confidently measure CBG levels in mouse samples, advancing their understanding of corticosteroid signaling pathways and their impact on health and disease.

Product Name:Mouse Corticosteroid Binding Globulin (CBG) ELISA Kit
Product Code:MODL00217
Size:96 Assays
Target:CBG
Synonyms:SERPINA6, Transcortin, Serpin A6, Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase, Antitrypsin)
Detection Method:Sandwich
Range:6.25-400ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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