The Mouse CORT (Corticosterone) ELISA Kit is designed for the quantitative detection of corticosterone levels in various mouse biological samples. As a vital hormone, corticosterone plays a substantial role in mouse's stress responses and energy metabolism processes. It is implicated in numerous physiological activities including the regulation of immune responses, inflammation, and glucose levels. Accurate measurement of corticosterone is paramount for understanding its role in stress-related disorders, endocrine health, metabolic issues, and inflammatory diseases in mice.
Assay Genie's Mouse CORT ELISA Kit provides excellent sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and is straightforward to use, making it an exceptional choice for both research and clinical applications. Place your trust in Assay Genie's Mouse CORT ELISA Kit for accurate and reliable quantification of this pivotal hormone in your studies.
Product Name:
Mouse CORT (Corticosterone) ELISA Kit
SKU:
AEES00019
Target:
Mouse CORT (Corticosterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
1.93 ng/mL
Detection range:
3.12-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
80-90
89-103
85-108
Average (%)
85
96
95
1:4
Range (%)
82-96
87-94
80-110
Average (%)
87
90
95
1:8
Range (%)
83-101
82-89
81-95
Average (%)
94
85
86
1:16
Range (%)
88-106
89-104
82-96
Average (%)
94
95
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
81-94
86
EDTA plasma (n=5)
87-99
91
Cell culture media (n=5)
84-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.44
80.37
185.44
21.23
82.33
187.01
Standard deviation
1.99
6.7
14.13
2.1
7.31
15.0
C V (%)
9.73
8.34
7.62
9.88
8.88
8.02
Sample type &Sample volume:
serum and plasma; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CORT concentrations in serum and plasma.
Specificity:
This kit recognizes CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.
